国外研究

Nature Genetics:细胞发育中泛基因组变化研究

作者: 来源:科学时报 2017-11-23 我要评论( )

在细胞发育过程中,调控细胞从前体细胞进化到成熟细胞的遗传因素是什么?三项大型研究在日前在线出版的《自然·遗传学》期刊上报告说,他们揭开了这个秘密。 不成熟细胞是指仍需要

在细胞发育过程中,调控细胞从前体细胞进化到成熟细胞的遗传因素是什么?三项大型研究在日前在线出版的《自然·遗传学》期刊上报告说,他们揭开了这个秘密。

不成熟细胞是指仍需要发育的细胞,而成熟细胞则是指已拥有特别功能的细胞。器官或组织的完整发育取决于不成熟细胞和成熟细胞之间的兼容性。迄今为止,科学家们对这种细胞在生长与特别功能之间的切换是如何进行的仍知之甚少。

Yoshihide Hayashizaki、John Mattick和Piero Carninci领导的三个独立研究小组,利用深度测序技术评估了细胞发育过程中的基因表达的泛基因组变化。Carninci小组发现了重复移动因子的泛基因组表达,他们鉴别出25万个以前未知的基因表达始点,以及这些重复因子在邻近基因全表达中的功能后果;Mattick小组得出了另外一个有趣的发现:他们鉴别出一种全新的进化保守片段RNAs,它们位于活跃基因的始点位置。这两项发现与Hayashizaki小组的工作结合起来,有助于全面理解细胞发育的动态控制过程,这个控制过程支持了不成熟生长细胞向成熟功能细胞的转化。

生物谷推荐原始出处:

Nature genetics 41, 859 (2009)doi:10.1038/ng0709-859a

Tiny RNAs associated with transcription start sites in animals

Ryan J Taft, Evgeny A Glazov, Nicole Cloonan, Cas Simons, Stuart Stephen, Geoffrey J Faulkner, Timo Lassmann, Alistair R R Forrest, Sean M Grimmond, Kate Schroder, Katharine Irvine, Takahiro Arakawa, Mari Nakamura, Atsutaka Kubosaki, Kengo Hayashida, Chika Kawazu, Mitsuyoshi Murata, Hiromi Nishiyori, Shiro Fukuda, Jun Kawai, Carsten O Daub, David A Hume, Harukazu Suzuki, Valerio Orlando, Piero Carninci, Yoshihide Hayashizaki & John S Mattick

It has been reported that relatively short RNAs of heterogeneous sizes are derived from sequences near the promoters of eukaryotic genes. As part of the FANTOM4 project, we have identified tiny RNAs with a modal length of 18 nt that map within -60 to +120 nt of transcription start sites (TSSs) in human, chicken and Drosophila. These transcription initiation RNAs (tiRNAs) are derived from sequences on the same strand as the TSS and are preferentially associated with G+C-rich promoters. The 5' ends of tiRNAs show peak density 10–30 nt downstream of TSSs, indicating that they are processed. tiRNAs are generally, although not exclusively, associated with highly expressed transcripts and sites of RNA polymerase II binding. We suggest that tiRNAs may be a general feature of transcription in metazoa and possibly all eukaryotes.

1 Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Australia.
2 Diamantina Institute for Cancer, Immunology and metabolic Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, Australia.
3 RIKEN Omics Science Center, RIKEN Yokohama Institute, Yokohama, Kanagawa, Japan.
4 The Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, Australia.
5 The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Roslin, UK.
6 Dulbecco Telethon Institute, IRCCS Santa Lucia at EBRI, Rome, Italy.
7 Dulbecco Telethon Institute, IGB CNR, Naples, Italy.
8 The FANTOM Consortium.
9 RIKEN Omics Science Center.
10 General organizers (of the small RNA group).

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Nature,amp,nbsp,Genetics,细胞,发育

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